Methods for isolation of entomopathogenic fungi from the soil environment

by Nicolai V. Meyling

Summary
Descriptions of methods and recommendation of laboratory procedures for the isolation of
soil borne entomopathogenic fungi (specifically Beauveria spp. and M. anisopliae) are
presented. For screening of occurrences of indigenous populations of entomopathogenic fungi
the insect bait method is recommended. Further recommendations are: 1) Collect sufficient
number of soil samples to cover the area of investigation; 2) if the bait method is used, apply
sufficient individuals of bait insects to each sample to increase the likelihood of isolating the
fungi present. Descriptions of isolation methods, statistical analyses of the data and
preparation of media and bait insects are given.

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