- that is, not updated for awhile.
Anyway, below is that post of mine for those interested...
Anyway, below is that post of mine for those interested...
Showing posts with label cordyceps. Show all posts
Showing posts with label cordyceps. Show all posts
Friday, 19 January 2018
My solid substrate for Tolypocladium / ElaphoCordyceps ophioglossoides
Like three weeks ago I posted my old ophioglossoides recipe to Mycosymbiotics' blog, but that blog seems to be quite dead now
Wednesday, 16 January 2013
[Wealth] North Cordyceps growing behind the twists and turns (20130111) - 北虫草种植背后的一波三折
这是一种原本野生的菌类物质，这种很多人不认识的菌为何曾卖到1000多元一斤?他原本是搞建筑的包工头，他尝试人工栽培这种菌类几度失败，扔掉几十万元之后，他能否靠栽种这种菌类，取得他期望中的财富?
This is an original wild mushrooms substances, why a lot of people do not know this kind of bacteria had sold for 1,000 yuan a pound? Originally engaged in the construction of the labor contractor, he tried the artificial cultivation of this fungus failed several times, throw out after several hundred thousand dollars, whether he can rely on planting this fungus, made in his expectations of wealth?
[Google translated]
Watch the video
This is an original wild mushrooms substances, why a lot of people do not know this kind of bacteria had sold for 1,000 yuan a pound? Originally engaged in the construction of the labor contractor, he tried the artificial cultivation of this fungus failed several times, throw out after several hundred thousand dollars, whether he can rely on planting this fungus, made in his expectations of wealth?
[Google translated]
Watch the video
Friday, 21 December 2012
North Cordyceps cultivation techniques (20121217) - 北虫草培育技术
冬虫夏草因为药用价值高，滋补功效好，而家喻户晓。有一种虫草，不仅价值可以和冬虫夏草相媲美，而且还可以人工规模化栽培。这就是北虫草。北虫草，学名叫蛹虫草。是冬虫夏草的一个亲姐妹，同属于真菌门，子囊菌纲，肉座菌目，麦角菌科，虫草菌属。是虫草菌属的模式种。北虫草刚收获时，样子有点像菌类，烘干后的北虫草更像草。是一种药、食两用真菌，可以全草入药，具有药用价值和滋补功能。 本期节目就向朋友们北虫草的人工培育技术。
Cordyceps because of high medicinal value, tonic effects, while the household. A Chinese caterpillar fungus, not only the value and Cordyceps comparable, but also artificial large-scale cultivation. This is North Cordyceps. North Cordyceps school called Cordyceps Militaris. A Cordyceps sisters, belong to the same fungal door, Ascomycetes, meat seat bacteria mesh, ergot Division Cordyceps genus. Is the type species of the genus Cordyceps. North Cordyceps recently harvested, looks a bit like a fungus, North Cordyceps more like dried grass. A drug, edible fungus, whole plant medicine, medicinal value and nourishing function. In this program to friends Cordyceps artificial breeding techniques.
[Google translated]
Watch the video
Cordyceps because of high medicinal value, tonic effects, while the household. A Chinese caterpillar fungus, not only the value and Cordyceps comparable, but also artificial large-scale cultivation. This is North Cordyceps. North Cordyceps school called Cordyceps Militaris. A Cordyceps sisters, belong to the same fungal door, Ascomycetes, meat seat bacteria mesh, ergot Division Cordyceps genus. Is the type species of the genus Cordyceps. North Cordyceps recently harvested, looks a bit like a fungus, North Cordyceps more like dried grass. A drug, edible fungus, whole plant medicine, medicinal value and nourishing function. In this program to friends Cordyceps artificial breeding techniques.
[Google translated]
Watch the video
Cordyceps Militaris artificial breeding techniques (20120612) - 蛹虫草人工培育技术
蛹虫草作为冬虫夏草的保健品生产替代品，广泛用于制药，深受消费者青睐。人工培育蛹虫草，不仅占地少，栽培周期短，而且经济效益好，是农村农民致富的好项目，为我国名贵药材产业化生产开辟了新途径。本期节目分六个培养阶段为您介绍蛹虫草的培育技术。
Cordyceps Militaris Cordyceps health product alternatives, are widely used in the pharmaceutical, favored by consumers. Artificial to cultivate Cordyceps Militaris, covering less cultivation period is short, and good economic returns, is a good project for rural farmers to get rich, and opened up new avenues for the industrial production of rare medicinal herbs. In this program, divided into six training stage Cordyceps Militaris cultivation techniques to introduce you to.
[Google translated]
Watch the video
Cordyceps Militaris Cordyceps health product alternatives, are widely used in the pharmaceutical, favored by consumers. Artificial to cultivate Cordyceps Militaris, covering less cultivation period is short, and good economic returns, is a good project for rural farmers to get rich, and opened up new avenues for the industrial production of rare medicinal herbs. In this program, divided into six training stage Cordyceps Militaris cultivation techniques to introduce you to.
[Google translated]
Watch the video
Friday, 7 December 2012
Some Cordyceps and Paecilomyces spp that have produced fruiting bodies in artificial conditions
1. Cordyceps militaris, 2. Cordyceps taii, 3. Cordyceps phecocephla, 4.
Cordyceps longissima, 5. Cordyceps bassiana, 6. Cordyceps oxycephala, 7.
Cordyceps gracilioides, 8. Cordyceps myrmecophila, 9. Cordyceps
formicarum, 10. Cordyceps formosana Kobayasi & Shimizu, 11. Cordyceps
brongniatii, 12. Cordyceps nutans, 13. Cordyceps bifusispora aff., 14.
Cordyceps gunnii var. minor
( 十七种虫草的子实体培育研究 Artificial culture of seventeen Cordyceps spp.
LI Chun-Ru, NAM Sung-Hee, GENG De-Gui, FAN Mei-Zhen, LI Zeng-Zhi )
Cordyceps sp. of Dr. Tapingkae (ทะพิงค์แก)
And (O)C. sobolifera
cultivated with the same method.
( www.tapingkae.in.th )
Paecilomyces/Isaria japonica/tenuipes, Cordyceps pruinosa (Ho Gyoung Kim et al .)
and fumosoroseus, takaomontana, sinclairii, ophioglossoides, etc.etc...
few more nice pictures ( from www.mushtech.co.kr )
( www.tapingkae.in.th )
Cordyceps/Ophiocordyceps sinensis
( Professors Shen Nan Ying 沈南英 and Ke Chuan-Kui 柯传奎 1980's - 2004? )
Semicultivated Cordyceps/Ophiocordyceps sinensis
( Chinavivers and Daniel Winkler )
Paecilomyces cicadae ( Chai Yiqiu 柴一秋 ), Cordyceps cicadae (WANG, Yongjun et al .)
Paecilomyces/Isaria japonica/tenuipes, Cordyceps pruinosa (Ho Gyoung Kim et al .)
and fumosoroseus, takaomontana, sinclairii, ophioglossoides, etc.etc...
few more nice pictures ( from www.mushtech.co.kr )
Tuesday, 4 December 2012
Himalayan Viagra: A Mushroom Rises High
It’s one of the most expensive and elusive herbs in the world, selling at up to 8,000€ a kilo. Chinese billionaires want it for its legendary powers as an aphrodisiac. Doctors prize its medical properties. Every spring, entire villages in the Himalayas empty as people go in search of the yarshagumba mushroom. But it’s a difficult task, fraught with danger. The yarshagumba only grows for a few weeks of the year at high altitudes in the mountains. Harvesters, which include young children, run the risk of altitude sickness and breathing problems. We examine the yarshagumba phenomenon, following the mushrooms’ journey from the peaks of the Himalayas to the markets of Asia.
Georges HALPERN, J.Y WU, John HOLLIDAY
Watch Video Himalayan Viagra A Mushroom Rises High at blinkx
Georges HALPERN, J.Y WU, John HOLLIDAY
Watch Video Himalayan Viagra A Mushroom Rises High at blinkx
Ophiocordyceps sinensis clip
《大美青海》三江源 走遍中国 - "Great Beauty of Qinghai" Three Rivers Across China 20110220
Chinese and English subtitles
Watch the video
Chinese and English subtitles
Watch the video
Monday, 3 December 2012
Thursday, 29 November 2012
Selective Media for Isolation of Entomogenous Fungi
(from: Bioassays of Entomopathogenic Microbes and Nematodes CABI)
Veen’s agar medium (1 l) (Veen and Ferron, 1966)
35 g Mycological agar (Difco) or 10 g Oxoid neutralized soya peptone, 10 g
dextrose, 15 g No. 1. agar (or Bacto-agar), 1 g chloramphenicol (store 4°C),
and 0.5 g cycloheximide (= Actidione; store 4°C). Add 1 l distilled water, stir,
and cover. Autoclave for 10–15 min at 18–20 psi. Cool to c. 52°C and pour
plates in laminar flow cabinet.
Oatmeal dodine agar (Beilharz et al., 1982)
1. Antibiotic stock solution: add 4 g penicillin G (Sigma) and 10g streptomycin
sulphate (Sigma) to 40 ml sterile distilled water under sterile conditions.
Store at 4°C.
2. Crystal violet stock solution: add 0.1 g crystal violet (Sigma) to 200 ml distilled
water. Store in the dark.
3. Add 17.5 g oatmeal agar (Difco) and 2.5 g agar (Fisons) slowly to 0.5 l
distilled water while stirring vigorously and heat to boil.
4. Add 0.5 ml of the fungicide dodine (N-dodecylguanidine monoacetate;
Cyprex 65WP, American Cyanamid Co.) and 5 ml crystal violet stock solution
to the medium.
5. Autoclave for 20 min at 15 psi.
6. Allow medium to cool to 50–55°C and add 2 ml of antibiotic stock solution
under sterile conditions.
7. Swirl flask well to ensure thorough mixing of compounds and pour while
warm. There should be enough media for twenty 9-cm diameter Petri
dishes.
Selective agar medium (1 l) (Kerry et al., 1993)
37.5 mg carbendazim, 37.5 mg thiabendazole, 75 mg rose bengal, 17.5 g
NaCl, 50 mg each of streptomycin sulphate, aureomycin and chloramphenicol,
3 ml Triton X-100, and 17 g corn meal agar (Oxoid) in 1 l distilled water.
This medium is appropriate for selecting some Paecilomyces spp. and
Verticillium spp. from soil.
Paecilomyces lilicanus medium (Mitchell et al., 1987)
To prepare 1 l of medium, mix the following: 39 g PDA, 10–30 g NaCl, 1 g
Tergitol, 500 mg pentachloronitrobenzene, 500 mg benomyl, 100 mg streptomycin
sulphate, and 50 mg chlorotetracycline hydrochloride.
Wheat germ based selective agar medium (1 l) (Sneh, 1991)
1. Prepare an aqueous extract of wheat germ – mix 30 g wheat germ in 1 l
water, autoclave for 10 min and filter through four layers of cheesecloth.
2. Mix wheat germ extract (1 l) with 0.25 g chloramphenicol (heat stable) +
0.8 mg benlate (50% benomyl), 0.3 g dodine (65% n-dodecyl-guanidine
acetate), 10 mg crystal violet and 15 g agar.
3. Autoclave and pour into plates.
Copper-based selective agar medium (1 l) (Baath, 1991)
2% malt extract (Oxoid), 1.5% Agar (Difco) amended with 2–4 mg
CuSO4·5H2O per litre. Cordyceps militaris and Paecilomyces farinosus are
tolerant of high Cu levels (400 mg/L, followed by Metarhizium anisopliae
and Beauveria bassiana. Most other soil-borne fungi including
nematophagous species of Verticillium were less tolerant.
Veen’s agar medium (1 l) (Veen and Ferron, 1966)
35 g Mycological agar (Difco) or 10 g Oxoid neutralized soya peptone, 10 g
dextrose, 15 g No. 1. agar (or Bacto-agar), 1 g chloramphenicol (store 4°C),
and 0.5 g cycloheximide (= Actidione; store 4°C). Add 1 l distilled water, stir,
and cover. Autoclave for 10–15 min at 18–20 psi. Cool to c. 52°C and pour
plates in laminar flow cabinet.
Oatmeal dodine agar (Beilharz et al., 1982)
1. Antibiotic stock solution: add 4 g penicillin G (Sigma) and 10g streptomycin
sulphate (Sigma) to 40 ml sterile distilled water under sterile conditions.
Store at 4°C.
2. Crystal violet stock solution: add 0.1 g crystal violet (Sigma) to 200 ml distilled
water. Store in the dark.
3. Add 17.5 g oatmeal agar (Difco) and 2.5 g agar (Fisons) slowly to 0.5 l
distilled water while stirring vigorously and heat to boil.
4. Add 0.5 ml of the fungicide dodine (N-dodecylguanidine monoacetate;
Cyprex 65WP, American Cyanamid Co.) and 5 ml crystal violet stock solution
to the medium.
5. Autoclave for 20 min at 15 psi.
6. Allow medium to cool to 50–55°C and add 2 ml of antibiotic stock solution
under sterile conditions.
7. Swirl flask well to ensure thorough mixing of compounds and pour while
warm. There should be enough media for twenty 9-cm diameter Petri
dishes.
Selective agar medium (1 l) (Kerry et al., 1993)
37.5 mg carbendazim, 37.5 mg thiabendazole, 75 mg rose bengal, 17.5 g
NaCl, 50 mg each of streptomycin sulphate, aureomycin and chloramphenicol,
3 ml Triton X-100, and 17 g corn meal agar (Oxoid) in 1 l distilled water.
This medium is appropriate for selecting some Paecilomyces spp. and
Verticillium spp. from soil.
Paecilomyces lilicanus medium (Mitchell et al., 1987)
To prepare 1 l of medium, mix the following: 39 g PDA, 10–30 g NaCl, 1 g
Tergitol, 500 mg pentachloronitrobenzene, 500 mg benomyl, 100 mg streptomycin
sulphate, and 50 mg chlorotetracycline hydrochloride.
Wheat germ based selective agar medium (1 l) (Sneh, 1991)
1. Prepare an aqueous extract of wheat germ – mix 30 g wheat germ in 1 l
water, autoclave for 10 min and filter through four layers of cheesecloth.
2. Mix wheat germ extract (1 l) with 0.25 g chloramphenicol (heat stable) +
0.8 mg benlate (50% benomyl), 0.3 g dodine (65% n-dodecyl-guanidine
acetate), 10 mg crystal violet and 15 g agar.
3. Autoclave and pour into plates.
Copper-based selective agar medium (1 l) (Baath, 1991)
2% malt extract (Oxoid), 1.5% Agar (Difco) amended with 2–4 mg
CuSO4·5H2O per litre. Cordyceps militaris and Paecilomyces farinosus are
tolerant of high Cu levels (400 mg/L, followed by Metarhizium anisopliae
and Beauveria bassiana. Most other soil-borne fungi including
nematophagous species of Verticillium were less tolerant.
